钙结合蛋白D28K即用型免疫组化试剂盒-分析方法-资讯-生物在线

钙结合蛋白D28K即用型免疫组化试剂盒

作者:上海信裕生物科技有限公司 2023-04-25T00:00 (访问量:256)

 钙结合蛋白D28K即用型免疫组化试剂盒

 

英文名称 Calbindin Ready-To-Use IHC Kit
中文名称 钙结合蛋白D28K即用型免疫组化试剂盒
别    名 CAB27; CALB 1; CALB; CALB1; Calbindin 1 28kDa; Calbindin-D-28K; Calbindin D28; D 28K; D28K; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D dependent calcium binding protein avian-type; Vitamin D-dependent calcium-binding protein; CALB1_HUMAN.   
种    属 Human, Mouse, Rat
应用范围 Immunohistochemistry
适用样品基质 PFPE tissue
描述 IHC0106 is a ready-to-use IHC kit for staining of Calbindin. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
保存条件 Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍

产品组分及规格

编号

组分

规格

浓度

储存

1

PBS缓冲液干粉

2 L×2

20x

室温

2

抗原修复缓冲液

20 ml

100x

2-8

3

内源性过氧化物酶阻断剂

3 ml

RTU

2-8

4

封闭工作液

3 ml

RTU

2-8

5

一抗Calbindin Rabbit pAb

6 ml

RTU

2-8

6

二抗HRP-Goat anti-Rabbit IgG pAb

6 ml

RTU

2-8

7

DAB kit20×)显色液

0.3 ml

RTU

-20℃

8

 

DAB kit20×)稀释液

 

0.3 ml

RTU

-20

9

复染试剂

5 ml

RTU

室温

10

封片剂

5 ml

RTU

室温

11

对照大鼠肾,小鼠肾,人肾

3

RTU

室温

12

说明书

1

 

 

 

背景

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.

 

石蜡包埋组织的免疫组织化学方案

1. 脱蜡水化

石蜡切片置于新鲜二甲苯中浸泡脱蜡3次,每次15 min;依次置于不同浓度(100%95%90%80%70%)乙醇浸泡5 min,再置于蒸馏水洗涤5 min,重复3次。

2. 抗原修复

沸水浴修复:将100×抗原修复缓冲液(试剂2用蒸馏水稀释成抗原修复缓冲液,放入修复盒中并提前加热至95-100℃(注意盖好以防液体蒸发),然后将切片放入修复盒中,在沸水浴环境中保持外沸状态15 min,室温自然冷却;用PBS缓冲液(试剂1,将干粉溶解在2L蒸馏水中)清洗5 min,重复3次。

3. 阻断内源性过氧化物酶

用吸水纸吸去玻片上多余的液体,用免疫组化笔在组织周围画圈,加入2-4内源性过氧化物酶阻断剂(试剂3,室温下置于湿盒中孵育15 min,用PBS洗涤5 min,重复3次。

4. 血清封闭

用吸水纸吸去玻片上多余的液体,加入2-4封闭工作液(试剂4,置于湿盒内37℃封闭20 min,以减少非

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