Calcein-AM

MCE 国际站：Calcein-AM

产品活性：Calcein AM，中文名为该黄绿素乙酰氧基甲指，具细胞膜渗透性，可轻易进入到细胞内，Calcein AM 身并无荧光，进入细胞后被细胞中内源性酯酶水解生成具有强负电荷的不能通透细胞膜的极性分子钙黄绿素 (Calcein)，从而被滞留在细胞内。而 Calcein 可发出强绿色荧光，因此常与 Propidium Iodide 用于细胞活力/毒力检测，激发/发射波长：494/515 nm。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: General Protocol

1.Preparation of Calcein AM working solution
Preparation of 5 mM stock solution with DMSO.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Calcein AM working solution according to the actual situation.

2.Cell staining
2.1 Suspension cells（6-well plate）
a.Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 30-45 minutes.
c.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or fluorescence microplate readers.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-45 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or fluorescence microplate readers.

Storage
-20°C
Protect from light

Precautions
1.Please adjust the concentration of Calcein AM working solution according to the actual situation.
2.This product is for R&D use only, not for drug, household, or other uses.
3.For your safety and health, please wear a lab coat and disposable gloves to operate.

In Vivo: Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability.

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