Opti-MEM® I Reduced Serum Medium

基本信息

产品名称：Opti-MEM I Reduced Serum Medium

货号：31985070

品牌：Gibgo

规格：500ml

储存条件：2-8℃

用途：细胞培养

描述

Opti-MEM® Reduced-Serum Medium is an improved MinimalEssential Medium (MEM) that allows for a reduction of FetalBovine Serumsupplementation by at least 50% with no change to growth rateor morphology. Opti-MEM® is also recommended for use with cationic lipidtransfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be usedwith a variety of suspension and adherent mammalian cells, including Sp2, AE-1,CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a varietyof Gibco® Opti-MEM® modificationsfor a range of cell culture applications.

This Opti-MEM® is modified as follows:

The complete formulation is confidential. For moreinformation, please contact TechnicalServices.

Gibco® Opti-MEM® is a unique media that contains insulin,transferrin, hypoxanthine, thymidine, and trace elements. These additionalcomponents allow for a reduction in serum supplementation by at least 50%.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animaltherapeutic use. Uses other than the intended use may be a violation of locallaw.

cGMP Manufacturing and Quality System
Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in GrandIsland, New York. The facility is registered with the FDA as a medical devicemanufacturer and is certified to ISO 13485 and ISO 9001 standards. For supplychain continuity, Life Technologiesoffers an identical Gibco® Opti-MEM® productmade in our Scotland facility (31985-047). This facility is registered with theFDA as a medical device manufacturer and is certified to the ISO 13485standard.

Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g / L) andtherefore requires a 5-10% CO2 environment to maintain physiologicalpH.
     

常见问题解答

①   My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinizefor a shorter time or use less trypsin. Mycoplasma contamination could alsocause this problem. Segregate your culture and test for mycoplasma infection.Lastly, check for attachment factors in the medium.

② How much serum is in Opti-MEM™ I Medium?

None. The term “reduced serum” means that one can reducethe amount of serum they use with classical cell culture media. When workingwith Opti-MEM™ I medium, most cells that are routinely cultured inserum-supplemented medium may be transferred directly into Opti-MEM™ I mediumwith a minimum of 50% reduction in serum.