DNA损伤诱导转录因子3(DDIT3)重组蛋白说明书

Recombinant DNA Damage Inducible Transcript 3 (DDIT3) 

[DESCRIPTION]
Protein Names: DNA Damage Inducible Transcript 3

Synonyms:DDIT3, CHOP, CHOP10, GADD153

Species: Human

Size: 100µg

Source:Escherichia coli-derived

Subcellular Location :Nucleus

[PROPERTIES]

Residues: Met1~Ala169 with N-terminal His-Tag.

Grade & Purity: >95%, 21 kDa as determined by SDS-PAGE reducing conditions

Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method)

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 20.7 kDa

Predicted isoelectric point: 4.9

[PREPARATION]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37ºCfor 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCES]

The target protein is fused with N-terminal His-Tag, its sequence is listed below. 

MGHHHHHHSGSEF-MAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用