The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variability in size and shape, all neurons share common morphological features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system. The brain is known to contain billions of neurons, each being able to contact at least thousands of other neurons [2].

MN-h from ScienCell Research Laboratories are isolated from the neonate Day 2 C57BL/6 mouse brain hippocampus. MN-h were cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MN-h are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin III. MN-h are negative for mycoplasma, bacteria, yeast and fungi. MN-h are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use neuronal medium (NM, Cat. No. 1521) for the culture of mouse neurons in vitro.

Product Use

MN-h are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Parent, A. (1996) Neurons in Carpenters Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada.
[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd. ed., New York: Garland.