RPMI 1640 1X10L-细胞/细菌培养-试剂-生物在线
北京基尼亚生物技术有限公司
RPMI 1640 1X10L

RPMI 1640 1X10L

商家询价

产品名称: RPMI 1640 1X10L

英文名称: RPMI 1640 1X10L

产品编号: 23400062

产品价格: 0

产品产地: 进口

品牌商标: Life Technologies

更新时间: null

使用范围: null

北京基尼亚生物技术有限公司
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 Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool

This RPMI is modified as follows:

With Without
• L-glutamine • Sodium Bicarbonate
• Phenol Red  
• HEPES  


The complete formulation is available.

Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagles Minimal Essential Medium or Dulbeccos Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® RPMI 1640 product made in our Scotland facility (13018-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

内容和储存

 

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

详细说明

常用规格

Glutamine:

L-Glutamine 

Phenol Red Indicator:

Phenol Red 

Form:

Powder 

HEPES Buffer:

HEPES 

Serum Supplementation:

Standard Serum Supplementation 

Sodium Pyruvate Additive:

No Sodium Pyruvate 

Product Size:

10 L 

Shipping Condition:

Room Temperature 

Regulatory Statement:

For Research Use Only. Not for use in diagnostic procedures.