CHEK2 monoclonal antibody (M04), clone 4F10
产品名称： CHEK2 monoclonal antibody (M04), clone 4F10
英文名称： CHEK2 monoclonal antibody (M04), clone 4F10
产品编号： H00011200-M04
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant CHEK2.
- Immunogen:
- CHEK2 (AAH04207.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAAVL
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (85.14 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- CHEK2 monoclonal antibody (M04), clone 4F10 Western Blot analysis of CHEK2 expression in Hela NE ( Cat # L013V3 ).
- Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged CHEK2 is approximately 30ng/ml as a capture antibody.
- Protocol Download
- Entrez GeneID:
- 11200
- GeneBank Accession#:
- BC004207
- Protein Accession#:
- AAH04207.1
- Gene Name:
- CHEK2
- Gene Alias:
- CDS1,CHK2,HuCds1,LFS2,PP1425,RAD53
- Gene Description:
- CHK2 checkpoint homolog (S. pombe)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000028871,checkpoint-like protein CHK2,protein kinase CHK2,serine/threonine-protein kinase CHK2
- Gene Pathway
- Related Disease
- Adenocarcinoma
- Adenoma
- Ataxia telangiectasia
- Atherosclerosis
- Atherosclerosis
- Barrett Esophagus
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Breast Neoplasms, Male
- Carcinoma
- Carcinoma, Ductal
- Carcinoma, Ductal, Breast
- Carcinoma, Intraductal, Noninfiltrating
- Carcinoma, Lobular
- Carcinoma, Medullary
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Pancreatic Ductal
- Carcinoma, Squamous Cell