人转录误调节在实体肿瘤中的定量PCR芯片试剂盒描述：

          ScienCells GeneQuery™ Human Transcriptional Misregulation in Solid Tumors qPCR Array Kit (GQH-TMS) is designed to facilitate gene expression profiling of 88 key genes involved in six types of solid tumor development (prostate cancer, kidney cancer, thyroid cancer, neuroblastoma, carcinoid, and sarcomas). Brief examples of how included genes may be grouped according to function and tumor types are shown below:     

• Cell cycle regulation: CDK9, CDKN1A, CDKN1B
• Apoptosis and proliferation: TP53, RXRs, PPARG, MDM2
• Cellular invasion: MMP9, SPINT1, IL1R2, PLAU, PLAT, ZEB1
• Cell survival: BMI1, MYC, IGF1, MLF1
• Mutated transcription factors identified in
  • prostate cancer: ERG, ETV1, ETV4, ELK4
  • kidney cancer: TFE3
  • thyroid cancer: PAX8
  • neuroblastoma: MYCN
  • sarcomas: ATF1, FEV, WTI, DDIT3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Transcriptional Misregulation in Solid Tumors qPCR Array Plate Layout*

(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development

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