Product: Anti-Ku (p70/p80) mAb, clone 162
Cat. No.: MC-256 (100 μg)
Background:
The Ku autoantigen is a heterodimer of 70 kDa
(p70) and ~80 kDa (p80) proteins. The p70/p80
dimer is important for the function of a 460 kDa
DNA-dependent protein kinase that
phosphorylates certain transcription factors,
including Sp1, Oct-1, p53 and SV40 large T
antigen in vitro. Ku protein plays a role in cell
signaling, proliferation, DNA repair, replication,
transcriptional activation and apoptosis.
Molecular Weight of Antigen:
70 kDa and 80 kDa
Ig Isotype:
Mouse IgG2a
Immunogen:
Human B cell nuclei from plasmacytoid 2p68
cells.
Epitope:
A conformational epitope of p70/p80 dimer,
which is destroyed during Western blotting.
Species Reactivity:
This antibody is known to react with human,
monkey and Xenopus. Mouse and rat (immunoprecipitation
only). Does not react with bovine
and rabbit.
Positive Control:
Tonsil
Cellular Localization:
Nuclear
Format:
100 μg of monoclonal antibody at 200 μg/mL, in
10 mM PBS, pH 7.4, with protein stabilizer and
0.09 sodium azide. Purified from ascites fluid
by ammonium sulfate precipitation.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Flow cytometry

Immunofluorescence

Immunohistochemistry: (Formalin/paraffin)
Use antibody at 1-2 μg/mL for 30 minutes at
RT. [No special pretreatment is required for
staining formalin-fixed, paraffin-embedded
tissues]

Western blot: Not Suitable
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.