Product: Anti-Human Cyclooxygenase-2 (COX-2), clone AS66
Cat. No.: MC-451 (100 μg)
Background:
Cyclooxygenase (COX), also known as
prostaglandin H2 synthase and prostaglandin
endoperoxide synthase, is a key enzyme in the
conversion of arachidonic acid to prostaglandin
H2. Prostaglandin H2 is converted by other
enzymes into inflammatory mediators
prostaglandin (PG) D2, PGE2, PGF2a, PGI2
and Thromboxane A2. Thus, COX is a key
enzyme in the production of inflammatory agents
and is the target of intense research and drug
discovery activities. There are two enzymes of
COX, COX-1 and COX-2. COX-1 is
constitutively produced in many (most) cell types.
It is important in the gut for the production of
prostaglandins, which inhibit gastric secretion. It
can be induced in monocytes, macrophages and
other cells. It is induced as part of the
inflammatory response by IL-1beta and other
cytokines. It is induced by growth factors such
as EGF and PDGF. Expression is inhibited by
glucocorticosteroids such as cortisol and
dexamethasone. Lipopolysaccharide in
bacterial infections induces COX-2. COX-2 is
also found in elevated levels in synoviocytes
from rheumatoid arthritis patients. The
discomforts of inflammation such as pain and
swelling are largely due to the action of
prostaglandins produced by COX-2.
Species Reactivity:
Human. Other species not tested.
Ig Isotype:
Mouse IgG1/

Format:
100 μg of purified antibody at 1 mg/mL, provided
as 0.2 μm sterile filtered solution in PBS with
0.08% sodium azide.
Storage and Stability:
Antibodies should be stored at 4°C for short
periods. For longer storage aliquot and freeze
at -20°C.
Applications and Suggested Dilutions:

EIA

Flow Cytometry

Western blot: Use at 1-5 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.