The MEGAclear Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear Kit:

Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
High recovery from 1 ng to 500 g of input
Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
Quick protocol—purify RNA in less than 30 minutes

Using the MEGAclear Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.