GST MULTITRAP FF
产品名称： GST MULTITRAP FF
英文名称： GST MULTITRAP FF
产品编号： 28-4055-01
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983 点击查看
- 传真 : 点击查看
- 邮箱 : 342786402@qq.com
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GST MultiTrap FF
GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.
- 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* proteins.
- Highly reproducible well-to well and plate-to-plate results.
- High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
- Wells are prepacked with Glutathione Sepharose media for convenience.
- Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.
GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.
Purification of up to 0.5 mg of GST-tagged proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap filter plates. Direct purification of GST-tagged proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.
GST MultiTrap FF is prepacked with Glutathione Sepharose 4 fast flow, while GST MultiTrap 4B is prepacked with Glutathione Sepharose 4B.
GST MultiTrap FF — Technical Specifications
| Complete Packsize | 4 x 96-well filter plates |
| Storage Conditions | 4 - 30°C, 20% Ethanol |
| Centrifugation Force Recommended | 100 x g-500 x g |
| Centrifugation Force Max. | 700 x g |
| Media | |
|---|---|
| Ligand | Glutathione and 10-carbon linker arm |
| Average Particle Size | 90 µm |
| Matrix | Highly cross-linked 4% agarose |
| Binding Capacity/ml Chromatography Medium | ≈ 10 mg recombinant GST/ml medium1) |
| pH stability Working Range | 3-12 |
| pH stability Cleaning | 3-12 |
| Pressure/Flow Specification | base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Flow Velocity | <450 cm/h |
| Ligand Coupling Method | Epoxy activation |
| Storage Conditions | 4 to 30°C, 20% Ethanol |
| Chemical Stability | All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature |
| 1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |
| Column | |
|---|---|
| Complete Packsize | 4 x 96-well filter plates |
| Number of Wells | 96 |
| Sample Volume | 600 μl/well |
| Reproducibility Between Wells | ± 10% in yield |
| Material | Polypropylene (PP) and polyethylene (PE). |
| Dimensions | 127.8 x 85.5 x 30.6 mm |