CCNB1 polyclonal antibody
产品名称： CCNB1 polyclonal antibody
英文名称： CCNB1 polyclonal antibody
产品编号： PAB9943
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against full length recombinant CCNB1.
- Immunogen:
- Recombinant GST fusion protein corresponding to full length human CCNB1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse, Rat
- Form:
- Liquid
- Storage Buffer:
- In antiserum (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:2000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
Pines J, Hunter T.Nature. 1990 Aug 23;346(6286):760-3.
- 2.
- A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
Giordano A, Whyte P, Harlow E, Franza BR Jr, Beach D, Draetta G.Cell. 1989 Sep 8;58(5):981-90.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) shows detection of CCNB1 present in asynchronous HeLa cell lysates.
Comparison to a molecular weight marker indicates a band of ~55 KDa corresponding to human CCNB1 (arrowhead).
Approximately 50 ug of lysate was loaded on to a 7% SDS-PAGE gel for separation.
After transfer to nitrocellulose, the blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature.
Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG.
Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.
- Western Blot (Cell lysate)
- Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) antibody shows detection of human CCNB1 present in asynchronous HN30 cell lysates.
HN30 cells are from head and neck cancer tumors that over express CCNB1 and D1.
Comparison to a molecular weight marker indicates a band of ~62 KDa corresponding to the expected molecular weight for the protein (arrowhead).
The blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature.
Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG and chemiluminescence reagent with a 1-min exposure time.
Personal communication, Luca Cote, Temple University, Philadelphia, PA.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemical staining with CCNB1 polyclonal antibody (Cat # PAB9943) was diluted 1 : 500 to detect CCNB1 in human brain cerebellum tissue.
Tissue was formalin fixed and paraffin embedded.
No pre-treatment of sample was required.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
- Entrez GeneID:
- 891
- Protein Accession#:
- NP_114172;P14635
- Gene Name:
- CCNB1
- Gene Alias:
- CCNB
- Gene Description:
- cyclin B1
- Omim ID:
- 123836
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
- Other Designations:
- G2/mitotic-specific cyclin B1
- Interactome
- Gene Pathway