BACH1 polyclonal antibody
产品名称： BACH1 polyclonal antibody
英文名称： BACH1 polyclonal antibody
产品编号： PAB10027
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against partial recombinant BACH1.
- Immunogen:
- Recombinant protein corresponding to amino acids 92-104 of human BACH1.
- Host:
- Rabbit
- Reactivity:
- Chimpanzee, Human
- Specificity:
- Expect reactivity with isoform 1 and isoform 2 of BACH1.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:10000-1:44000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Analysis of the DNA substrate specificity of the human BACH1 helicase associated with breast cancer.
Gupta R, Sharma S, Sommers JA, Jin Z, Cantor SB, Brosh RM Jr.J Biol Chem. 2005 Jul 8;280(27):25450-60. Epub 2005 May 5.
- 2.
- Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, Cohn MA, Cantley LC, Gygi SP.Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12130-5. Epub 2004 Aug 9.
- 3.
- Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer.
Clapperton JA, Manke IA, Lowery DM, Ho T, Haire LF, Yaffe MB, Smerdon SJ.Nat Struct Mol Biol. 2004 Jun;11(6):512-8. Epub 2004 May 9.
- Applications
- Western Blot (Cell lysate)
- Western blot using BACH1 polyclonal antibody (Cat # PAB10027) shows detection of a band at~105 KDa (Lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead).
Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction.
Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,000.
Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at roomtemperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark
- Entrez GeneID:
- 571
- Protein Accession#:
- Q9BX63;NP_114432
- Gene Name:
- BACH1
- Gene Alias:
- -
- Gene Description:
- BTB and CNC homology 1, basic leucine zipper transcription factor 1
- Omim ID:
- 602751
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq
- Other Designations:
- BTB and CNC homology 1,OTTHUMP00000096564