Disease:

Histiocytic lymphoma 

Origin:

Lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA) 

Species:

Homo sapiens 

Tissue:

Blood 

Morphology:

Monocyte 

Properties:

Suspension 

Cytogenic data:

Patient:

Male, Caucasian, 37 yrs of age 

Medium:

RPMI1640 + 10% FBS 

Subculture:

Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 to 2 X 106 cells/ml. Culture cells at 5% CO2, 37°C. 

Medium Renewal:

Add fresh medium every 3 to 4 days. 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Isoenzymes:
 Not known 
DNA Profile (STR):
 Amelogenin: X ; CSF1PO: 12; D13S317: 10,12; D16S539: 12; D5S818: 12; D7S820: 9,11; THO1: 6, 9.3; TPOX: 8,11; vWA: 14, 15; 
Mutations in U937 Cells

Related cells by Organ:

CCRF-CEM 

K562 

LP-1 

MOLM-13 

RPMI-8226 

U937 

RAW 264.7


References for U937 cells:

1. Finamore, E., Vitiello, M., D'Isanto, M., Galdiero, E., Falanga, A., Kampanaraki, A., Raieta, K., and Galdiero, M. Evidence for IL-6 promoter nuclear activation in U937 cells stimulated with Salmonella enterica serovar Typhimurium porins. Eur Cytokine Netw, 20: 140-147, 2009. 

2. Kokkinou, I., Fragoulis, E. G., and Vassilacopoulou, D. The U937 macrophage cell line expresses enzymatically active L-Dopa decarboxylase. J Neuroimmunol, 216: 51-58, 2009. 

3. Liu, W. H. and Chang, L. S. Reactive oxygen species and p38 mitogen-activated protein kinase induce apoptotic death of U937 cells in response to Naja nigricollis toxin-gamma. J Cell Mol Med, 13: 1695-1705, 2009. 

4. Liu, Y., Geng, L., and Suo, Z. Differentiation effect of pyruvate and uridine on cultured U937-rho degrees cells. Ultrastruct Pathol, 33: 160-164, 2009. 

5. Marfe, G., Pucci, B., De Martino, L., Fiorito, F., Di Stefano, C., Indelicato, M., Aventaggiato, M., Russo, M. A., and Tafani, M. Heat-shock pretreatment inhibits sorbitol-induced apoptosis in K562, U937 and HeLa cells. Int J Cancer, 125: 2077-2085, 2009. 

6. Wang, J. H., Zhou, Y. J., Tian, L., and He, P. Phosphoinositide 3-kinase/Akt and nuclear factor-kappaB are involved in Staphylococcus aureus-induced apoptosis in U937 cells. Chin Med Sci J, 24: 231-235, 2009.