IL1B polyclonal antibody
产品名称: IL1B polyclonal antibody
英文名称: IL1B polyclonal antibody
产品编号: PAB11294
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against partial recombinant IL1B.
- Immunogen:
- Recombinant protein corresponding to 153 amino acids of human IL1B.
- Host:
- Rabbit
- Reactivity:
- Human
- Specificity:
- The MW of the recombinant IL-1beta was 17,000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:200-1:1000)
Western Blot (1:100-1:200)
Immunocytochemistry (1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(1:100-1:200)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes.
Thornberry NA, Bull HG, Calaycay JR, Chapman KT, Howard AD, Kostura MJ, Miller DK, Molineaux SM, Weidner JR, Aunins J, et al..Nature. 1992 Apr 30;356(6372):768-74.
- 2.
- Sandwich ELISA formats designed to detect 17 kDa IL-1 beta significantly underestimate 35 kDa IL-1 beta.
Herzyk DJ, Berger AE, Allen JN, Wewers MD.J Immunol Methods. 1992 Apr 8;148(1-2):243-54.
- 3.
- Molecular cloning of the interleukin-1 beta converting enzyme.
Cerretti DP, Kozlosky CJ, Mosley B, Nelson N, Van Ness K, Greenstreet TA, March CJ, Kronheim SR, Druck T, Cannizzaro LA, et al..Science. 1992 Apr 3;256(5053):97-100.
- Applications
- Western Blot (Recombinant protein)
- Immunoblotting of IL1B polyclonal antibody (Cat # PAB11294) was used at a 1 : 200 dilution incubated 1 h at room temperature to detect dog IL1B by Western blot.
Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL1B respectively.
The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown).
Detection occurred using a 1 : 3,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
- Application Image
- Western Blot (Recombinant protein)
- enlarge
- Immunohistochemistry
- Immunoprecipitation
- ELISA
- Entrez GeneID:
- 3553
- Gene Name:
- IL1B
- Gene Alias:
- IL-1,IL1-BETA,IL1F2
- Gene Description:
- interleukin 1, beta
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq
- Other Designations:
- catabolin,preinterleukin 1 beta,pro-interleukin-1-beta
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