PKLR (N-term) Peptide
产品名称： PKLR (N-term) Peptide
英文名称： PKLR (N-term) Peptide
产品编号： P2138
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PKLR.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PKLR.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Liver pyruvate kinase polymorphisms are associated with type 2 diabetes in northern European Caucasians.
Wang H, Chu W, Das SK, Ren Q, Hasstedt SJ, Elbein SC.Diabetes. 2002 Sep;51(9):2861-5.
- 2.
- PKLR- GBA region shows almost complete linkage disequilibrium over 70 kb in a set of worldwide populations.
Mateu E, Perez-Lezaun A, Martinez-Arias R, Andres A, Valles M, Bertranpetit J, Calafell F.Hum Genet. 2002 Jun;110(6):532-44. Epub 2002 May 22.
- 3.
- Structure and function of human erythrocyte pyruvate kinase. Molecular basis of nonspherocytic hemolytic anemia.
Valentini G, Chiarelli LR, Fortin R, Dolzan M, Galizzi A, Abraham DJ, Wang C, Bianchi P, Zanella A, Mattevi A.J Biol Chem. 2002 Jun 28;277(26):23807-14. Epub 2002 Apr 17.
- Applications
- Western Blot (Cell lysate)
- The PKLR polyclonal antibody (Cat # PAB4572) is used in Western blot to detect PKLR in NIH/3T3 cell lysate .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKLR polyclonal antibody (Cat # PAB4572) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Entrez GeneID:
- 5313
- Protein Accession#:
- NP_000289;P30613
- Gene Name:
- PKLR
- Gene Alias:
- PK1,PKL,PKR,PKRL,RPK
- Gene Description:
- pyruvate kinase, liver and RBC
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- R-type/L-type pyruvate kinase,pyruvate kinase 1,pyruvate kinase isozyme R/L,pyruvate kinase type L,pyruvate kinase, liver and RBC type,pyruvate kinase, liver and blood cell,red cell/liver pyruvate kinase